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No Differences in Outcome after Intracytoplasmic Sperm Injection with Fresh or with Frozen-thawed Epididymal Spermatozoa. (PDF, 66 KB) Human Reproduction, 1999. An Azoospermic Man with a do novo Point Mutation in the Y-chromosome Gene USP9Y. (PDF, 381 KB) Nature Genetics, 1999.

For a woman to conceive, certain things have to happen: vaginal intercourse must take place around the time when an egg is released from her ovary; the system that produces eggs has to be working at optimum levels; and her hormones must be balanced.[54] For women, problems with fertilisation arise mainly from either structural problems in the Fallopian tube or uterus or problems releasing eggs. Ultrastructure of Human Sperm in Men with Congenital Absence of the Vas Deferens: Clinical Implications. (PDF, 2 MB) Fertility and Sterility, 1992.

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After fertilization, implantation may then take place in the uterus. Common causes of infertility of females include: ovulation problems (e.g. polycystic ovarian syndrome, PCOS, the leading reason why women present to fertility clinics due to anovulatory infertility.[58]) tubal blockage pelvic inflammatory disease caused by infections like tuberculosis age-related factors uterine problems previous tubal ligation endometriosis advanced maternal age immune infertility Males[edit] The main cause of male infertility is low semen quality. Torsion: Is a common problem affecting fertility that is caused by a supportive tissue abnormality which allows the testes to twist inside the scrotum which is characterized by extreme swelling.  Torsion pinches the blood vessels that feed the testes shut which causes testicular damage.  If emergency surgery is not performed to untwist the testes, torsion can seriously impair fertility and cause permanent infertility if both testes twist. Intracytoplasmic Sperm Injection. (PDF, 4 MB) Chapter 47 from textbook Current Therapy in Equine Reproduction, 2006. The sperm must provide the zygote with DNA, centrioles, and activation factor for the embryo to develop.

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Other causes include Y chromosome microdeletion, a depletion of chromosome material that impedes or severely decreases spermatozoa production. Existing definitions of infertility lack uniformity, rendering comparisons in prevalence between countries or over time problematic. If too many embryos develop, one or more can be removed. These include advancing maternal age, severe male factor infertility (whereby ICSI can be used to fertilize the egg), and endometriosis, amongst many others. The content of counselling may differ depending on the concerned couple and the existing treatment options. If a blockage is present, surgery may be necessary.

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Serum gonadotrophins (follicle-stimulating hormone and luteinising hormone) on Day2-3 especially in irregular periods. (N. Conventional IVF vs ICSI (Intra Cytoplasmic Sperm Injection) for Patients Requiring Microsurgical Epididymal Sperm Aspiration (MESA). (PDF, 4 MB) Chapter from textbook, Frontiers in Endocrinology: Perspectives on Assisted Reproduction, 1994. Luteinized unruptured follicle syndrome can be prevented by the administration of hCG (10,000 IU IM) once the follicle reaches 23-24 mm in diameter. As they respond to hormones the same way as they would do in the uterus, that is by growing and shedding cyclically, endometriosis can cause both fallopian tube and ovarian scarring. Such tissue growth and its surgical removal can cause scarring and prevent fertilization.  Uterine and cervical causes  Problems in the uterus or cervix may also affect fertility. This technique involves an injection of a single healthy sperm directly injected into mature egg.